Use of Capillary Electrophoresis for the Detection of Single-Residue Substitutions in Peptide Mapping

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Abstract
Capillary electrophoresis (CE) can provide an orthogonal separation to the reversed phase HPLC commonly used for peptide mapping, thus ensuring that all possible amino acid substitutions can be detected. the selectivity of a reversed phase separation is based on the relative hydrophobicity of the components while that of CE is derived from charge, mass, diameter and viscosity. Since CE also provides so many options for effecting and modifying a peptide separation, it is useful to define one possible systematic approach to methods development. the application of such an approach to the tryptic peptides of cytochrome c yields a method suitable for the identification of differences of a single residue between homologous proteins from different species.