New laboratory scale purification of β-amanitin from American Amanita phalloides
- 5 September 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (18) , 3786-3789
- https://doi.org/10.1021/bi00611a018
Abstract
A modern laboratory scale method for purifying .beta.-amanitin from the mushroom A. phalloides is described. The purification involves solubilizing the toxin from dried mushrooms, removing the lipids by chloroform extraction, desalting on Amberlite XAD-4, adsorption chromatography on Sephadex LH-20 at acidic and neutral pH, and ion-exchange chromatography on QAE-Sephadex. The resulting .beta.-amanitin is homogeneous by TLC and is biologically active.This publication has 3 references indexed in Scilit:
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- AMATOXINS AND PHALLOTOXINS IN AMANITA-SPECIES OF NORTHEASTERN UNITED-STATES1977
- Über die Giftstoffe des Knollenblätterpilzes. IVEuropean Journal of Organic Chemistry, 1938