Evaluation of immunoglobulin purification methods and their impact on quality and yield of antigen-specific antibodies
Open Access
- 14 July 2008
- journal article
- research article
- Published by Springer Nature in Malaria Journal
- Vol. 7 (1) , 129
- https://doi.org/10.1186/1475-2875-7-129
Abstract
Antibodies are the main effectors against malaria blood-stage parasites. Evaluation of functional activities in immune sera from Phase 2a/b vaccine trials may provide invaluable information in the search for immune correlates of protection. However, the presence of anti-malarial-drugs, improper collection/storage conditions or concomitant immune responses against other pathogens can contribute to non-specific anti-parasite activities when the sera/plasma are tested in vitro. Purification of immunoglobulin is a standard approach for reducing such non-specific background activities, but the purification method itself can alter the quality and yield of recovered Ag-specific antibodies.Keywords
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