The Effect of Ringing on Cytokinin Activity in Shoots of the Grape Vine

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Abstract
The effects of ringing (girdling) on cytokinin levels in shoots of grape vines were investigated. Treated and control plants both contained two active fractions; the major constituent (Fraction A) was retained by cationic exchangers, whereas the other (Fraction B) was not. Two days after ringing, the level of Fraction A dropped to a value never less than half that of the control level, but did not continue to fall after the second day, nor did it rise to values greater than those in control plants during the 9 days of the experiment. The level of Fraction B also dropped on the second day after ringing, and thereafter Fraction B could not be detected. The possible identities of the fractions are discussed.

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