Alteration of immunolysis reaction on liposome membrane by various cholesterol analogues.
- 1 January 1988
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 36 (10) , 4192-4198
- https://doi.org/10.1248/cpb.36.4192
Abstract
Complement-mediated immunolysis was employed to examine the effect of incorporation ofvarious cholesterol analogues, having a terminal hydroxyl group or terminal primary amine groupat the 3-position of the cholestene nucleus, into the bilayer membrane of haptenated reverse-phaseevaporation vesicles. An enhancement of immunolysis was observed when triethoxycholesterol (analogue II) was incorporated, while no measurable change was detected when the chain length ofthe substituted groups in hydroxy cholesterols was shorter than that of analogue II. However, foramino cholesterol analogues, a remarkable decrease in immunolysis was seen. These results mayarise from changes of membrane properties such as bilayer fluidity, lateral hapten mobility and complement fixation.Keywords
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