The Effect of Trypsin on Hemagglutination by Murine Encephalomyelitis Virus (GDVII)

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Abstract
The GDVII strain of murine encephalomyelitis virus has recently been shown to cause agglutination of human red cells at 4 C (1). This reaction did not occur at room temperature or at 37 C, and the agglutination occurring at 4 C was dispersed when the temperature was raised. This reversal of hemagglutination was due to elution of the virus but was not accompanied by a destruction of cell receptors as occurs with viruses of the mumps-influenza group. Of a variety of red blood cells examined, only human cells were found susceptible to agglutination by GDVII virus. The report of Rheims, Dodd and Hudson (2), that treatment of human group O cells with trypsin increased their agglutinability by mouse lung infected with PR8 influenza virus, suggested the present study. In anticipation of the results to be reported, it may be said that exposure of the cells to trypsin did not affect hemagglutination by GDVII virus.