Electroporation-induced transformation of Escherichia coli: evaluation of a square waveform pulse
- 1 February 1991
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 12 (2) , 39-42
- https://doi.org/10.1111/j.1472-765x.1991.tb00498.x
Abstract
Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a sinusoidal waveform of exponential decaying pulse.Keywords
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