Isolation of Allergenically Active Cytochrome c from Kentucky Blue Grass Pollen
- 1 January 1981
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 65 (4) , 367-376
- https://doi.org/10.1159/000232778
Abstract
An allergen, designated as Kentucky blue grass KBG-cytochrome c, was isolated from the nondialysable constituents (R) of the aqueous extract of KBG pollen by a combination of gel filtration and preparative isoelectrofocussing techniques. The allergen had an absorption spectrum characteristic of heme protein and had a molecular weight corresponding to 12,000 daltons. It was a basic protein with a pI value of 9.9 and contained all the common amino acids. KBG-cytochrome c elicited immediate hypersensitivity cutaneous reactions in individuals allergic to KBG pollen.Keywords
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