COMPARISON OF MICROBIOLOGICAL AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC ASSAYS OF THE NEW CEPHALOSPORIN CEFROXADINE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 3  (3) , 179-182
Abstract
A method employing high pressure liquid chromatography [HPLC] was developed for the new cephalosporin cefroxadine (CGP 9000). This uses Lichrosorb RP-8 5 .mu.m as the stationary phase and 2 mM phosphoric acid mixed with methanol in the ratio 72:28 for the mobile phase. The retention time is 6.5 min. The procedure has a correlation with microbiological assay of r = 0.968 for sera after oral dosage and r = 0.944 after i.v. doses. Correlations in sera from each [human] individual has ranged from 0.957-0.998. A protein binding of 5% was found for a concentration of 10 .mu.g/ml and of 7.5% at 2 .mu.g/ml. The HPLC method had a recovery of 92.5 at a low level of 2 .mu.g/ml and 95% at 10 .mu.g/ml.

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