Sample Profiles and Temperature Transients in Preparative Gas Chromatography

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Abstract
This paper gives results of investigating some of the parameters involved in the operation of a preparative gas-chromatographic unit. An elution profile for a nonabsorbed sample was noted across the column bed. Elution-rate differences indicate that the carrier gas flow rate is somewhat faster near the column wall than in the central bed. However, in at least some cases, overloading samples of absorbing solutes gave more rapid elution through the central axial portion of the column than in the near-wall portion of the column bed. Apparently, heat transfer and solute equilibrium change with concentration and temperature differences across the column bed, and both steady-state and transient temperature differences between the axis and wall have such pronounced effects that carrier gas velocity differences across the bed are of secondary importance in 1-in. I.D. columns.