Effect of Methylprednisolone on the Oxidative Burst Activity, Adhesion Molecules and Clinical Outcome Following Open Heart Surgery
- 1 January 1997
- journal article
- clinical trial
- Published by Taylor & Francis in Scandinavian Cardiovascular Journal
- Vol. 31 (5) , 283-288
- https://doi.org/10.3109/14017439709069549
Abstract
Following cardiac surgery with cardiopulmonary bypass (CPB), activated granulocytes may be involved with ischaemia/ reperfusion injury. The purpose of this study was to investigate whether steroids could reduce the oxidative burst activity of granulocytes, the expression of adhesion molecules on granulocytes and improve clinical outcome. Sixteen patients undergoing open heart surgery participated in the study. Eight were randomized to receive methylprednisolone (30 mg/kg intravenously) at the start of anaesthesia while eight patients served as a control group. The oxidative burst was measured flow cytometrically using 123-dihydrorhodamine. A panel of adhesion molecules was measured using monoclonal antibodies. Following CPB the oxidative burst activity and the expression of the adhesion molecule L-selectin more than doubled compared to initial values. There was no difference between the steroid group and the control group regarding the expression of adhesion molecules or the oxidative burst activity. In the steroid group the fluid gain during extracorporeal circulation (ECC) was 683 ml (median) compared to 1488 ml in the control group. Steroids prevented hyperthermia in the postoperative period but did not improve the weaning from the ventilator or reduce the stay in the intensive-care unit. In conclusion, treatment with steroids prevented hyperthermia following open heart surgery with CPB and reduced capillary leak during ECC. Methylprednisolone, however, did not reduce the oxidative burst activity or the expression of adhesion molecules on granulocytes following CPB.Keywords
This publication has 22 references indexed in Scilit:
- Role of Transcriptional Activation of IκBα in Mediation of Immunosuppression by GlucocorticoidsScience, 1995
- Influence of steroids on complement and cytokine generation after cardiopulmonary bypassThe Annals of Thoracic Surgery, 1995
- A chimeric IgG4 monoclonal antibody directed against CD18 reduces infarct size in a primate model of myocardial ischemia and reperfusionJournal of the American College of Cardiology, 1995
- Redistribution of granulocytes in patients after major surgical stressAPMIS, 1994
- Heparin-coated bypass circuits reduce pulmonary injuryThe Annals of Thoracic Surgery, 1993
- Inflammatory response to cardiopulmonary bypassThe Annals of Thoracic Surgery, 1993
- Leukocyte depletion in a neonatal model of cardiac surgeryThe Annals of Thoracic Surgery, 1993
- Flow cytometric measurement of the respiratory burst activity of phagocytes using dihydrorhodamine 123Journal of Immunological Methods, 1991
- Neutrophil-activating protein 1/interleukin 8 stimulates the binding activity of the leukocyte adhesion receptor CD11b/CD18 on human neutrophils.The Journal of Experimental Medicine, 1990
- Natural Killer Cell Activity and Lymphocyte Function During and After Coronary Artery Bypass Grafting in Relation to the Endocrine Stress ResponseAnesthesiology, 1987