QUANTITATIVE CHROMATOGRAPHIC METHODS FOR THE STUDY OF ENZYMIC TRANSPEPTIDATION REACTIONS

1 January 1955

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology

Vol. 33 (1) , 416-427
https://doi.org/10.1139/y55-055

Abstract

A colorimetric ninhydrin method has been applied to the quantitative estimation of amino acids and peptides on paper chromatograms. This method is suitable for the analysis of enzymic transpeptidation reactions. A procedure for the purification of filter paper for chromatography has been developed.

Keywords

PURIFICATION
CHROMATOGRAPHY
FILTER
COLORIMETRIC
PROCEDURE
NINHYDRIN
CHROMATOGRAMS
CHROMATOGRAPHIC
AMINO
ENZYMIC

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