Hydrocortisone elicits the effect of clomiphene citrate on luteinizing hormone-releasing hormone release in vitro

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Abstract
The effect of hydrocortisone on the release of luteinizing hormone (LH) and LHRH in response to clomiphene citrate (clomiphene) were examined in a sequential double chamber perifusion system by perifusing the mediobasal hypothalami (MBH) and/or pituitaries excised from normal female rats in diestrus. When the MBH and the pituitary were perifused in sequence with medium containing 5 .times. 10-6 M hydrocortisone, a significant release in LH (100-150% increase, P < 0.01-P < 0.05) was observed 40 min after the administration of 3 .times. 10-8 mol clomiphene. Clomiphene had no effect on LH release from the pituitary when perifused in series with the MBH without basal hydrocortisone infusion. Administration of clomiphene did not cause a significant increase in LH from the pituitary perifused alone, with or without medium containing hydrocortisone. The concentration of LHRH in the efflux was significantly increased 40 min after clomiphene administration when MBH was perifused with medium containing hydrocortisone, whereas clomiphene had no effect when perifused with medium only. Hydrocortisone apparently stimulates the effect of clomiphene on LHRH release from the hypothalamus, which in turn induces LH release from the pituitary.