Theoretical and practical considerations in the determination of aneurine (vitamin B1) with special reference to the recovery factor

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Abstract
Analysis of factors affecting the "recovery" of added thiamine to extracts showed that variation of sample and errors in volume measurements are considerable. Combination of thiamine during digestion, possible effects at the oxidation stage, and interference with the emission of fluorescent light in the fluorimeter are considered. Practical results are given and investigation of effects in the fluorimeter cell is included in some detail. Losses that take place in base-exchange methods are mentioned. The remarkable stability of the thiochrome method is shown.

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