Effects of pH, Ca2+, temperature, and protease pretreatment on interkingdom fusion
- 1 July 1979
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 5 (4) , 529-536
- https://doi.org/10.1007/bf01538886
Abstract
The incubation of carrot protoplasts and culturedXenopus cells in a protease solution has been shown to enhance their subsequent interkingdom fusion by a high pH/high Ca2+ method. The effects of Ca2+ concentration, pH, and temperature on the frequency of heterokaryon formation have also been studied. Potentially viable heterokaryons have been repeatedly produced at high frequencies (consistently >10%), far exceeding those so far achieved in PEG-mediated fusion. Cell aggregates are readily dispersed after this method of fusion, permitting the accurate estimation of fusion frequencies.This publication has 18 references indexed in Scilit:
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