The effects of substrate, temperature, and photoperiod on conidiationof Pyrenophora tritici-repentis
- 1 February 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 55 (3) , 254-259
- https://doi.org/10.1139/b77-035
Abstract
Fifteen isolates of Pyrenophora tritici-repentis were used to determine the effects of substrate, temperature, and photoperiod on growth and conidiation. Conidiation was achieved with seven isolates when either 15% V-8 juice agar (1.5 g/l CaCO3 and 15 g/l agar) or autoclaved leaves of wheat or wheatgrass (Agropyron dasystachyum (Hook.) Scribn.) were used as substrates. However, V-8 juice agar provided the most consistent and abundant conidiation. Conidiophore production was observed over a temperature range of 10 to 31 °C while conidia were produced from 10 to 25 °C. Optimal conidiation on 15% V-8 juice agar occurred at about 21 °C. Twenty-five different photoperiods were tested and conidiophores were formed with all regimens containing alight period. Conidia were produced with 1 to 21 h of light (4790 lx) per day with an optimum at 12 ± 1 h at 21 °C on 15% V-8 juice agar.Keywords
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