Detection of t(11;22)(q24;ql2) Translocation Breakpoint in Paraffin-embedded Tissue of the Ewingʼs Sarcoma Family by Nested Reverse Transcription-Polymerase Chain Reaction
- 1 June 1996
- journal article
- Published by Wolters Kluwer Health in Diagnostic Molecular Pathology
- Vol. 5 (2) , 107-113
- https://doi.org/10.1097/00019606-199606000-00005
Abstract
Tumors of the Ewing's sarcoma family often present a major diagnostic challenge for the pathologist. In recent years, significant progress has been made in identifying characteristic chromosomal rearrangements associated with certain solid tumors. More than 85% of Ewing's sarcoma and related tumors present a specific t(11;22) (q24;q12) balanced translocation, which generates a fusion transcript of the EWS gene and the FLI-1 gene. The cloning of the t(11;22)(q24;q12) breakpoint has raised the possibility of using a reverse transcription-polymerase chain reaction (RT-PCR) based assay as a diagnostic tool. We report an improvement of the established method, which currently depends on fresh or snap-frozen tissue, so that it is possible to use formalin-fixed, paraffin-embedded tissue as a source of RNA. The described nested RT-PCR assay enables the pathologist to investigate retrospectively archival tumor samples or to confirm the diagnosis in cases where no fresh or frozen material is available.Keywords
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