Light microscopic observations of Echinostoma caproni metacercariae during in vitro excystation

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Abstract
Light microscopy was used to study excystation of Echinostoma caproni metacercariae in various media. Optimal excystation was observed in an alkaline trypsin-bile salts medium (TB) at 40 ± 1°C. The percentage of excystation in TB medium declined following either acid pepsin or acid saline pretreatment. Excystation rarely occurred in either trypsin or bile salts alone. Dissolution of the outer cyst occurred in TB medium thus facilitating excystation. Activation of the larva within the inner cyst led to its eventual release through an aperture in the mucoid plug region. Excystation of this species was facilitated by both intrinsic and extrinsic factors. There were considerable differences in excystation events between this species and the related allopatric species, E. trivolvis

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