Vascular permeability responses and the role of prostaglandin E2 in an experimental allergic inflammation of air pouch type in rats
Open Access
- 1 May 1986
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 87 (4) , 751-756
- https://doi.org/10.1111/j.1476-5381.1986.tb14593.x
Abstract
1 Rats were sensitized with azobenzene arsonate-conjugated acetyl bovine serum albumin. An allergic inflammation was induced in the preformed air pouch in the dorsum of the sensitized rats by injecting the antigen dissolved in a 2% sodium carboxymethyl cellulose solution into the air pouch. 2 Time course changes of vascular permeability, accumulated pouch fluid volume and prostaglandin E2 (PGE2) levels in the pouch fluid were compared in sensitized and non-sensitized rats to characterize the allergic inflammatory reaction. 3 Effects of three cyclo-oxygenase inhibitors (indomethacin, diclofenac sodium and tiaprofenic acid) on vascular permeability and accumulated pouch fluid volume 4 and 24 h after the immunological challenge injection were examined to elucidate a possible role of PGE2 in the inflammatory response. 4 Four h after initiating the allergic reaction, although the level of PGE2 in the pouch fluid reached a high level, the vascular permeability response, measured over the period 3.5–4 h, was not suppressed by treatment with the three cyclo-oxygenase inhibitors and neither was the pouch fluid volume measured over the period 0–4 h. However, vascular permeability and accumulated pouch fluid volume at 24 h were suppressed by the cyclo-oxygenase inhibitors in a dose-dependent manner. 5 These observations suggest that in this model, endogenous PGE2 does not affect oedema formation measured at 4 h. However, oedema formation measured at 24 h may be dependent on PGE2 generation.This publication has 22 references indexed in Scilit:
- Reduction by dexamethasone of chemotactic activity in inflammatory exudatesEuropean Journal of Pharmacology, 1984
- Dexamethasone inhibits generation in inflammatory sites of the chemotactic activity attributable to leukotriene B4Biochemical and Biophysical Research Communications, 1984
- Arachidonate Metabolites in Acute and Chronic Allergic Air Pouch Inflammation in Rats and the Anti-Inflammatory Effects of Indomethacin and DexamethasoneInternational Archives of Allergy and Immunology, 1984
- Correlation of Leukocyte Accumulation with Chemotactic Activity in the Exudate of an Allergic Air-Pouch InflammationInternational Archives of Allergy and Immunology, 1983
- A Possible Role of Arachidonate Metabolism in Allergic Air Pouch Inflammation in RatsInternational Archives of Allergy and Immunology, 1982
- Delayed-Type Hypersensitivity as Revealed on the Footpads of Mice to Azobenzenearsonate-Acetyl Bovine Serum AlbuminInternational Archives of Allergy and Immunology, 1981
- Action of nonsteroidal, anti-inflammatory drugs on human and rat peripheral leucocyte migration in vitro.Annals of the Rheumatic Diseases, 1977
- Interactions of bradykinin, prostaglandin E1, 5-hydroxytryptamine, histamine and adenosine-5′-triphosphate on the dye leakage response in rat skinJournal of Pharmacy and Pharmacology, 1976
- Prostaglandins as Potentiators of Increased Vascular Permeability in InflammationNature, 1973