Preparation and purification of L-(.+-.)-5-formyl-5,6,7,8-tetrahydrofolic acid

Abstract
Reinvestigation of the conversion of folic acid to leucovorin [L-(.+-.)-5-CHO-THF] (L-(.+-.)-5-formyl-5,6,7,8-tetrahydrofolic acid) led to improved methods for the synthesis of the drug [a rescue factor in methotrexate cancer chemotherapy] which is suitable for clinical use. Methods were developed for the chromatographic and nonchromatographic purification of less pure samples of L-(.+-.)-5-CHO-THF.

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