An Investigation Into A Dye-Binding Procedure For Measuring Available Lysine In Raw And Cooked Beans

Abstract
Raw and cooked (two different methods of cooking) blackeye (Vigna unguiculata) and red kidney (Phaseolus vulgaris) beans were analysed for crude protein and available lysine (dye-binding with acid orange 12 dye, before and after treatment with propionic an hydride). Results of these investigations showed that open cooking in water for 3 hunder mild heat treatment showed slight increase in crude protein and available lysine content while dehulling, grinding and steaming for 1 h showed slight loss of crude protein and available lysine. Sieving in the dye-binding method was shown to be important.

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