Abstract
A cooperative study, involving 12 federal, state, local, and private laboratories throughout the country, was conducted to evaluate six methods of preparing and using the methylene blue stain for the direct microscopic count of bacteria in milk. Three of the six methods were found superior, yielding significantly higher bacteria counts, at the same time providing greater ease in counting. These three methods are: Levine and Black's acid-and-water-free stain; North's aniline oil stain; and Anderson's polychrome stain.

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