Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages
- 18 December 1995
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 377 (2) , 227-231
- https://doi.org/10.1016/0014-5793(95)01348-2
Abstract
We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected. The phage genome encodes a fusion of single-chain antibody to the C-terminal domain of gIIIp, rendering the phage non-infective. The antigen fused to the N-terminal domains of gIIIp is encoded in the same phage genome. Antigen and antibody fusion interact with each other in the periplasm of the phage-producing cell, restoring infectivity. This system has a very low background and will allow simultaneous randomisation of antibody and antigen.Keywords
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