The isolation by cataphoresis of two different oxyhæmoglobins from the blood of some animals

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Abstract
Many observers have found specific differences in the properties of hæmoglobins from different species (Barcroft, 1928). These differences are due—as Barcroft states—to the globins, whereas the hematin is probably the same in all hæmoglobins (Barcroft, 1928). Another important question was raised by Bohr (1892). This author advanced the hyppthesis that more than one hæmoglobin may be present in the same animal. Bohr's hypothesis was also supported by Hoppe-Seyler's investigations (Hoppe-Seyler). Crystallographic observations on hæmoglobins by Reichert and Brown (1909) showed that it is possible to obtain different crystals are dimorphous or polymorphous in may species of animals. A. K. Boor, criticising the findings of Reichert and Brown, states (Boor, 1930) that the conditions of crystallisation were not uniform. According to Boor, various organic impurities might have influenced the crystal habit, as well as the crystal system and form. Boor prepared oxyhæmoglobin by mixing washed red corpuscles with aluminium cream, followed by filtration in a refrigerator. The crystals were formed from the pure filtrate by adding a suitable amount of ethyl alcohol and maintaining a temperature of 0°C. or lower. Only one kind of crystal of horse oxyhæmoglobin and of horse carbon monoxide hæmoglobin is reported, while dog oxhæmoglobin was found to be represented by two forms, though only one form of dog carbon monoxide hæmoglobin was observed. According to Boor, every case of dual form is doubtful, and he suggests that there is only one hæmoglobin for each species.

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