Isoelectric Focusing and Separation of the Subcomponents of Lactoperoxidase.
- 1 January 1967
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 21 (1) , 271-278
- https://doi.org/10.3891/acta.chem.scand.21-0271
Abstract
The existence of a pronounced heterogeneity of lactoperoxidase was confirmed by the method of isoelectric focusing of ampholxtes. Six subtractions were demonstrated and their specific activities and spectrophotometric properties were investigated. The heterogeneity pattern obtained by isoelectric focusing was compared to the results obtained by moving boundary electrophoresis, disc electrophoresis on polyacrylamide, and sedimentation analysis. An explanation of the heterogeneity has been suggested.Keywords
This publication has 5 references indexed in Scilit:
- Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. V. Separation of Myoglobins and Studies on their Electro-Chemical Differences.Acta Chemica Scandinavica, 1967
- Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. IV. Further Studies on the Resolving Power in Connection with Separation of Myoglobins.Acta Chemica Scandinavica, 1966
- On the Heterogeneity of Beef Heart Cytochrome c. IV. Isoelectric Fractionation by Electrolysis in a Natural pH-Gradient.Acta Chemica Scandinavica, 1966
- The Heterogeneity of Lactoperoxidase.Acta Chemica Scandinavica, 1965
- The Isolation And Purification Of Lactoperoxidase By Ion Exchange ChromatographyJournal of Biological Chemistry, 1957