Analysis of Blood Transketolase Activity

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Abstract
The determination of blood transketolase (TK) activity is based on the incubation of lysed erythrocytes with and without added thiamine pyrophosphate (TPP). The increased TK activity resulting from the addition of TPP coenzyme is inversely related to thiamine depletion. The proposed method employs glycylglycine as buffer and is a macro adaptation of the Dreyfus ultramicro assay. With the procedure described, precision is approximately 1%, TK activity is linear with time and volume of whole blood analyzed, calibration curves follow Beer’s law, and results are corrected for hematocrit. The upper limit of normal at the 95% confidence limits is 86.1 mU. per 1. per min. and the corresponding “in vitro” TPP effect is 17.4%.

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