Amino Acid Sequence of Japanese Horseshoe Crab (Tachypleus tridentatus) Coagulogen B Chain: Completion of the Coagulogen Sequence12
- 1 May 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 95 (5) , 1445-1457
- https://doi.org/10.1093/oxfordjournals.jbchem.a134752
Abstract
The complete amino acid sequence of the B chain derived from Tachypleus tridentatus coagulogen was determined. It consisted of a total of 129 amino acid residues with a NH2-terminal glycine and COOH-terminal phenylalanine. Sequence studies of the whole B chain and the fragments obtained from the digests with trypsin, α-chymotrypsin, thermolysin and Staphylococcal protease V8 showed the following sequence: 1 10 20 NH2-G-F-S-I-F-S-H-H-P-V-F-R-E-C-G-K-Y-E-C-R- T-V-R-P-E-H-S-R-C-Y-N-F-P-P-F-T-H-F-K-L- E-C-P-V-S-T-R-D-C-E-P-V-F-G-Y-T-V-A-G-E- F-R-V-I-V-Q-A-P-R-A-G-F-R-Q-C-V-W-Q-H-K- C-R-F-G-S-N-S-C-G-Y-N-G-R-C-T-Q-Q-R-S-V- V-R-L-V-T-Y-N-L-E-K-D-G-F-L-C-E-S-F-R-T- C-C-G-C-P-C-R-S-F-OH These structural studies of the B chain and the previously established amino acid sequences of the A chain and peptide C derived from T. tridentatus coagulogen, now make it possible to complete the whole sequence of coagulogen consisting of 175 amino acid residues with the molecular weight of 19,723.Keywords
This publication has 1 reference indexed in Scilit:
- A Clottable Protein (Coagulogen) of Horseshoe Crab Hemocytes. Structural Change of Its Polypeptide Chain during Gel Formation1The Journal of Biochemistry, 1976