Cloning and sequencing of the cDNA encoding rice elongation factor 1β′

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Abstract
A cDNA clone coding for rice elongation factor 1β′(EF‐1β′) was isolated from a rice anther cDNA library. The clone, named RB′, was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF‐1β′ shows homology to the C‐terminal half of Artemia salina EF‐1β (59%) and human EF‐1β (63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemia salina EF‐1β and EF‐σ, human EF‐1β and silkworm EF‐1β′.