TOLERANCE INDUCTION IN MATURING B CELLS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (3) , 283-290
Abstract
A 2-stage tissue culture system was used to test the concept of clonal abortion as a mechanism for tolerance induction in [mouse] B [bone marrow-derived] cells. In the 1st stage, neonatal spleen cells or bone marrow cells were cultured for 72 h under conditions in which B-cell neogenesis occurred. Haptens coupled to various carriers were introduced during this stage. Following this culture phase, the cells were washed and their competence to respond to haptenated-POL [polymerized flagellin] was measured in microcultures where feedback effects were minimized. The results indicated that immature B cells were specially susceptible to tolerance but that the conditions under which hapten was presented were also important in determining the outcome of the cell-antigen encounter.