Nuclear matrix DNA from chicken erythrocytes contains beta-globin gene sequences.
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (2) , 304-307
- https://doi.org/10.1073/pnas.81.2.304
Abstract
Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purine nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of .beta.-globin gene sequences as assayed by dot hybridization with 32P-labeled nick-translated pHB1001. EM of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.This publication has 36 references indexed in Scilit:
- A protein that preferentially binds Drosophila satellite DNA.Proceedings of the National Academy of Sciences, 1979
- ELECTRON MICROSCOPE VISUALIZATION OF CHROMATIN AND OTHER DNA-PROTEIN COMPLEXESAnnual Review of Biophysics and Bioengineering, 1978
- Intragenic DNA spacers interrupt the ovalbumin geneProceedings of the National Academy of Sciences, 1978
- The role of nonhistone chromosomal proteins in the interaction of prostate chromatin with androgen · receptor complexBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- Characterization of a DNA binding protein from rat liver chromatin which decreases during growthBiochemistry, 1978
- Glucocorticoid-Receptor Complex Binds to Nonhistone Protein and DNA in Rat Liver Chromatin1The Journal of Biochemistry, 1978
- Identification of nonhistone chromatin proteins in chromatin subunits.Proceedings of the National Academy of Sciences, 1976
- Regulation of in vitro mRNA transcription by a fraction of chromosomal proteins.Journal of Biological Chemistry, 1975
- Immunospecificity of chromatin nonhistone protein—DNA complexes in normal and neoplastic growthFEBS Letters, 1974
- Modification of ribonucleic acid synthesis in isolated rat liver nuclei by low salt concentrations and specific divalent cationsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969