Cloning and expression in Escherichia coli of a cDNA encoding a developmentally regulated Ca2+‐binding protein from Dictyostelium discoideum
- 10 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (3) , 342-346
- https://doi.org/10.1016/0014-5793(95)00272-b
Abstract
We have cloned a full‐length cDNA from Dictyostelium discoideum which encodes a new Ca2+‐binding protein. The deduced protein (termed CBP1) is composed of 156 amino acids and contains four consensus metal‐ligating loop sequences found in helix‐loop‐helix motifs of many Ca2+‐binding proteins. When expressed in bacteria as a GST fusion protein, CBP1 binds Ca2+ in a 45Ca2+ overlay assay. CBP1 exhibits little amino acid sequence homology with Dictyostelium calmodulin or calfumirin‐1 (CAF‐1) except in the putative Ca2+‐binding regions. Moreover, unlike calmodulin and CAF‐1 expression, CBP1 mRNA is expressed preferentially during the multicellular stages of development.Keywords
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