RADIOIMMUNOASSAY FOR TRIIODOTHYRONINE IN HUMAN SERUM

1 March 1973

journal article
Published by Oxford University Press (OUP) in Acta Endocrinologica

Vol. 72 (3) , 464-474
https://doi.org/10.1530/acta.0.0720464

Abstract

A radioimmunoassay for triiodothyronine (T₃) is demonstrated. Details of the T₃ antibody binding reaction are discussed and the statistical data of the assay system are presented. Plasma samples were first extracted by a simple ion exchange method and the extracts then tested by radioimmunoassay. The mean normal concentration was 1.35 ± 0.29 ng/ml. Later, direct measurements in serum samples were performed after partial inhibition of T₃ binding to TBG by merthiolate and high pH buffer. After correction for the residual non-specific binding a mean normal plasma level of 1.2 ± 0.3 ng/ml was obtained.

Keywords

ANTIBODY
TRIIODOTHYRONINE
BINDING
RADIOIMMUNOASSAY
PARTIAL
LATER
BUFFER
MERTHIOLATE
TBG
SERUM

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