Peptide sequencing identifies MSS1, a modulator of HIV Tat‐mediated transactivation, as subunit 7 of the 26 S protease
- 1 June 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 323 (3) , 276-278
- https://doi.org/10.1016/0014-5793(93)81356-5
Abstract
Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1Keywords
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