Cytochemical localization of ?-galactosidase in resident and inflammatory peritoneal macrophages from C57BL mice

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Abstract
A cytochemical method for the detection of α-galactosidase (β-Gase) in mouse peritoneal macrophages was used to study the ultrastructural localization of this enzyme in these cells. It was found that the reaction product for β-Gase was localized in the perinuclear cisternae, the endoplasmic reticulum, the Golgi complex, lysosomes, vesicles and on the cell surface of peritoneal macrophages from untreated C57BL mice. When examined by X-ray microanalysis the crystalline reaction product was found to contain bromine, an element present in the indolyl substrate which was used to identify β-Gase. Injection of Proprionibacterium acnes (P. acnes) intraperitoneally or BCG intravenously caused a visible loss in β-Gase from all the organelles and from the cell surface of the macrophages.

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