Antibody Against Neoplastic Plasma Cells

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Abstract
Antigenic differences between mouse myeloma cells and normal mouse lymph node cells were investigated by comparing the reaction pattern of rabbit antisera prepared against cellular components of myelomas of BALB/c mice and of antisera prepared against lymph node cells. The fluorochromasia cytotoxicity test was used. Cells of six individual myeloma lines were used for immunization and cytotoxicity tests. Anti-myeloma sera cytotoxic to myeloma cells of all the lines, yet not cytotoxic to normal lymph node cells, were obtained. The myeloma-cytotoxic antibodies were removed readily by absorption with myeloma sediments, but not at all with sediments of thymus, liver and kidney. Sediments of lymph node and spleen were effective absorbants only when they were used in extremely large amounts. These results indicate the presence of surface antigens on myeloma cells which are not present on lymphocytes, or liver and kidney cells. These antigens are probably present also on normal plasma cells, which comprise a small fraction of the total cell population in the lymph nodes and spleen of normal mice. Although anti-myeloma sera were prepared against individual myeloma lines, all the antisera were cytotoxic to myeloma cells of all the lines. No antigens truly specific to particular lines could be detected even after absorption of antisera with other myelomas. Anti-lymph node sera cytotoxic to lymph node and spleen cells, yet not cytotoxic to myeloma cells, were obtained after appropriate absorption. The lymphocyte-cytotoxic antibodies were removed readily by treatment with lymph node, spleen and thymus sediments. Sediments of myeloma cells and of liver and kidney cells were much less effective, indicating an antigenic difference between lymphocytes and the latter cells.

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