Studies on the Genetic Multiplicity of a Gene in Yeast Cells, I Characteristics of the Induced Mutations by Subcritical Temperature and Ultraviolet Light

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Abstract
The duplex nature of gene mutation of ad locus in yeast cells was studied by using subcritical temperature for one-hit type action and using ultraviolet light for two-hit action. The relative frequencies of whole colony mutation and fractional colony (sectored) mutation induced by these 2 agents are strikingly different: 59% whole, 23% fractional and 18% undefined for subcritical temperature; 14% whole, 72% fractional and 13% undefined for ultraviolet light. If it is assumed that subcritical temperature causes changes in both strands of a gene-DNA molecule and ultra-violet in one of the 2 strands by a single event, the results observed might be interpreted to indicate a genetic multiplicity of 2 of the gene.