Oil synthesis in vitro in microsomal membranes from developing cotyledons of Linum usitatissimum L.

1 January 1985

journal article
research article
Published by Springer Nature in Planta

Vol. 164 (1) , 101-104
https://doi.org/10.1007/bf00391032

Abstract

Microsomal preparations from developing linseed (Linum usitatissimum L.) cotyledons catalyzed i) acyl exchange between acyl-CoA and position 2 of sn-phosphatidylcholine, ii) acylation of sn-glycerol 3-phosphate to yield phosphatidic acid, and iii) the utilisation of phosphatidic acid in the production of diacylglycerol and triacylglycerol. Selectivity studies for C₁₈ acyl species of acyl-CoA indicated a bias for the channelling of oleate to phosphatidylcholine for, presumably, its desaturation, and the utilisation of the polyunsaturated fatty-acid products in the acyl-CoA pool for phosphatidic acid and subsequent triacylglycerol synthesis. The microsomal preparations were capable of returning glycerol backbone with associated acyl components to phosphatidylcholine from diacylglycerol where it may be further enriched with polyunsaturated C₁₈ acids by desaturation. The acyl quality in linolenate-rich oilseeds appears to be under similar control to that found in linoleate-rich species.

Keywords

This publication has 13 references indexed in Scilit:

The regulation of the fatty-acid composition of the triacylglycerols in microsomal preparations from avocado mesocarp and the developing cotyledons of safflower
Planta, 1985
The synthesis of linoleate and phosphatidic acid and its relationship to oil production in the microsomes of developing seeds of safflower (Carthamus tinctorius, L. var. Gila)
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
Some evidence for the reversibility of the cholinephosphotransferasecatalysed reaction in developing linseed cotyledons in vivo
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
The role of the acyl-CoA pool in the synthesis of polyunsaturated 18-carbon fatty acids and triacylglycerol production in the microsomes of developing safflower seeds
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
Catalase stimulates linoleate desaturase activity in microsomes from developing linseed cotyledons
FEBS Letters, 1981
The biosynthesis of linoleate and α-linolenate in homogenates from developing soya bean cotyledons
Plant Science Letters, 1980
A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
Analytical Biochemistry, 1976
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Analytical Biochemistry, 1976
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Canadian Journal of Biochemistry and Physiology, 1959
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Canadian Journal of Biochemistry and Physiology, 1959