Expression and modulation of surface antigens in cultured rat glomerular visceral epithelial cells.
Open Access
- 1 November 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 37 (11) , 1675-1687
- https://doi.org/10.1177/37.11.2809176
Abstract
This study, using immunocytochemical light and electron microscopy techniques, characterizes the distribution of three antibodies bound to the surface of rat glomerular visceral epithelial cells (GEC) in culture, and tests their ability to redistribute corresponding antigens under conditions appropriate for antigenic modulation (antigen disappearance). At 4 degrees C or after fixation, anti-renal tubular brush border vesicle (BBV) IgG bound diffusely to the surface of GEC and to coated pits. Anti-gp330 IgG had a discrete distribution on the surface of GEC and reacted with coated pits. Anti-podocalyxin IgG was bound diffusely to the surface of GEC but not to coated pits. At 37 degrees C, anti-BBV IgG induced marked redistribution of immune complexes with both shedding and internalization. Anti-gp330 IgG induced weaker redistribution, with internalization of immune complexes predominating. Anti-podocalyxin IgG induced rapid redistribution of immune complexes and antigenic modulation but minimal internalization. Experiments of differential redistribution indicated that anti-BBV IgG modulated the expression of both gp330 and podocalyxin; anti-gp330 IgG had a weaker effect on BBV antigens and podocalyxin; and anti-podocalyxin failed to redistribute BBV antigens or gp330. The relevance of these immunocytochemical studies of antibody-cell surface antigen interaction in cultured GEC to understanding the pathogenesis of Heymann glomerulonephritis (HG) is discussed.This publication has 38 references indexed in Scilit:
- A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell LinesThe Journal of Immunology, 1979
- TRANSPORT OF ORGANIC IONS IN RENAL CORTICAL LUMINAL AND ANTILUMINAL MEMBRANE-VESICLES1979
- Experimental glomerulonephritis in the isolated perfused rat kidney.Journal of Clinical Investigation, 1978
- Isolation and characterization of rat glomerular epithelial cells in vitroKidney International, 1978
- An association between actin and the major histocompatibility antigen H–2Nature, 1978
- Cell surface shedding—The phenomenon and its possible significanceJournal of Theoretical Biology, 1976
- Immunoglobulins and alloantigens on the surface of lymphoid cellsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1975
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966
- The hydrolysis of rabbit γ-globulin and antibodies with crystalline papainBiochemical Journal, 1959
- Production of Nephrotic Syndrome in Rats by Freund's Adjuvants and Rat Kidney Suspensions.Experimental Biology and Medicine, 1959