Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.

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Abstract
The nucleotide sequence was determined of a cDNA [complementary DNA] clone that codes for the 60,000-dalton .gamma. subunit of T. californica acetylcholine receptor. The length of the cDNA clone is 2010 base pairs. The 5'' and 3'' untranslated regions have respective lengths of 31 and 461 base pairs. Data suggest that the putative polyadenylylation consensus sequence A-A-T-A-A-A may not be required for polyadenylylation of the mRNA corresponding to the cDNA clone described in this study. From the DNA sequence data, the amino acid sequence of the .gamma. subunit was deduced. The subunit is composed of 489 amino acids giving a molecular mass of 56,600 daltons. The deduced amino acid sequence data also indicate the presence of a 17-amino acid extension or signal peptide on this subunit. From these data, structural predictions for the .gamma. subunit are made such as potential membrane-spanning regions, possible asparagine-linked glycosylation sites and the assignment of regions of the protein to the extracellular, internal and cytoplasmic domains of the lipid bilayer.