A New Sensitive and Rapid Automated Fluorometric Assay for Detection of Natural Killer Activity Using Carboxyfluorescein Diacetate
- 1 March 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 12 (1) , 145-157
- https://doi.org/10.1080/01971529108055062
Abstract
An automated fluorometric assay using carboxyfluorescein diacetate (CFDA) has been applied for the sensitive and rapid detection of natural killer (NK) activity. The lysis of target cells by NK cells was quantified by measuring the amount of CFDA released into the supernatant of culture wells with the aid of an automated microfluorometer. Both sensitivity and specificity of the presented method were higher than the 51Cr release assay. Moreover, the detection of human NK activity against K562 target cells required only 2 hrs, compared to 4 hrs in the standard 51Cr release assay.Keywords
This publication has 4 references indexed in Scilit:
- Automated fluorometric assay for T cell cytotoxicityJournal of Immunological Methods, 1988
- A fluorescence NK assay using flow cytometryJournal of Immunological Methods, 1986
- Carboxyfluorescein fluorochromasia assays. I. Non-radio actively labeled cell mediated lympholysisJournal of Immunological Methods, 1980
- Target-Effector Interaction in the Natural Killer (NK) Cell SystemThe Journal of Immunology, 1978