The activity of the gluconate‐H+ symporter of Schizosaccharomyces pombe cells is down‐regulated by d‐glucose and exogenous cAMP

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Abstract
Schizosaccharomyces pombe cells take up d‐gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol‐containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of d‐glucose as well as its non‐metabolizable analogues to glycerol‐grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down‐regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by d‐glucose but also by extracellular cAMP even in the absence of the cAMP‐dependent protein kinase (PKA1).