Sequential G- to R-banding for high resolution chromosome analysis
- 1 May 1987
- journal article
- research article
- Published by Springer Nature in Human Genetics
- Vol. 76 (1) , 37-39
- https://doi.org/10.1007/bf00283047
Abstract
A sequential staining protocol for revealing first G-and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.Keywords
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