Increased Detection of Acid-Injured Escherichia coli O157:H7 in Autoclaved Apple Cider by Using Nonselective Repair on Trypticase Soy Agar

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Abstract
Three different acid-resistant strains of Escherichia coli O157:H7 were inoculated individually and as a cocktail into sterile apple cider (pH 3.2) at a level of approximately 105 cells per ml and incubated at 2°C. Samples were plated on Trypticase soy agar (TSA), violet red bile agar (VRBA), sorbitol MacConkey agar (SMA), and Petrifilm E. coli count plates (Petrifilm) at 24-h intervals. Repair of acid-injured cells was assessed by surface plating cider samples on TSA and allowing a 2-h room-temperature incubation period followed by overlaying with double-strength VRBA or SMA. Since SMA is a surface plate medium, the repair procedure was modified by overlaying SMA with Trypticase soy broth after 2 h of room-temperature incubation. Populations of all three strains and the cocktail of strains decreased rapidly in apple cider and approached undetectable levels within 72 h. At 24 and 48 h, 98.4% and >99% of the E. coli populations were injured, respectively. Repair procedures significantly (α = 0.05) increase...

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