IDENTIFICATION OF BACILLUS ANTHRACIS BY API TESTS

Abstract
API and morphological tests were examinted for their ability to distinguish between 37 B. anthracis strains (virulent and avirulent) and 194 strains of closely related Bacillus spp. (B. cereus, B. mycoides and B. thuringiensis). In addition, 34 strains of B. anthracis and 4 of B. cereus were tested by several other methods that included capsule formation, ability to grow on a selective medium and sensitivity to phage. It was found that virulent strains of B. anthracis were easily separated from the closely related Bacillus spp. by most of the test methods; but separation of slightly virulent and avirulent strains of B. anthracis from the closely related species could be done only by API and phage-sensitivity tests.

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