Fluorescence quenching at interfaces and the permeation of acrylamide and iodide across phospholipid bilayers
- 29 October 2001
- journal article
- Published by Wiley
- Vol. 330 (2) , 129-132
- https://doi.org/10.1016/0014-5793(93)80257-u
Abstract
Studies of fluorescence quenching in membrane proteins are complicated by the fact that the barrier effect of the bilayer towards the quenchers is not known with precision. Our studies show that (a) both acrylamide and iodide can permeate the membrane at comparable rates, (b) when quenchers are added externally to a vesicle suspension, the apparent Stern-Volmer quenching constants for the same fluorophores are lower in the inner than in the outer aqueous compartments, and (c) at least some non-polar fluorophores embedded in the bilayer are quenched by iodide, but not by acrylamide.Keywords
This publication has 13 references indexed in Scilit:
- The role of charge and hydrophobicity in peptide-lipid interaction: a comparative study based on tryptophan fluorescence measurements combined with the use of aqueous and hydrophobic quenchersBiochemistry, 1990
- Tryptophan fluorescence of mitochondrial complex III reconstituted in phosphatidylcholine bilayersArchives of Biochemistry and Biophysics, 1987
- Melittin-phospholipid interaction studied by employing the single tryptophan residue as an intrinsic fluorescent probeBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Fluorescence quenching studies with proteinsAnalytical Biochemistry, 1981
- Tryptophan fluorescence of (Na+ + K+)-ATPase as a tool for study of the enzyme mechanismBiochimica et Biophysica Acta (BBA) - Enzymology, 1978
- Fluorescence quenching of indole and model micelle systemsThe Journal of Physical Chemistry, 1976
- X-Ray Diffraction Studies of Lecithin Bimolecular Leaflets with Incorporated Fluorescent ProbesProceedings of the National Academy of Sciences, 1972
- Solute perturbation of protein fluorescence. Quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ionBiochemistry, 1971
- Lipid composition and permeability of liposomesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1968
- Diffusion of univalent ions across the lamellae of swollen phospholipidsJournal of Molecular Biology, 1965