Generation of deletions through a cis-acting mutation in plasmid pC194

1 March 1985

journal article
research article
Published by Springer Nature in Molecular Genetics and Genomics

Vol. 198 (3) , 432-436
https://doi.org/10.1007/bf00332935

Abstract

When plasmid pC194-1 is ligated to pBR322 to generate plasmid pHV15-1, deletions occur with high frequency within the joined pBR322 DNA. Generation of deletions is recE4 independent, and occurs in B. subtilis with a 1,000-fold higher frequency than in Escherichia coli. In the hybrid plasmid pVH15-1, deletion end-points are not at random, but at defined locations within pBR322. We propose that the base alteration, characterizing pC194-1, has stabilized within the plasmid a stem/loop structure, which acts as a deletion generator.

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