MEASUREMENT OF PROPRANOLOL, 4-HYDROXYPROPRANOLOL AND PROPRANOLOL GLYCOL IN HUMAN-PLASMA

  • 1 January 1979
    • journal article
    • research article
    • Vol. 24  (1) , 3-12
Abstract
An HPLC [high pressure liquid chromatography] method for the quantitative determination of propranolol, 4-hydroxypropranolol and propranolol glycol in human plasma is described. The limits of sensitivity for the method were 1 ng/nl propranolol, 5 ng/ml 4-hydroxypropranolol and 1 ng/ml propranolol glycol. Data obtained from 6 healthy volunteers given a single 160 mg oral dose of propranolol revealed mean (.+-. SEM [standard error of the mean]) peak plasma levels of 31 .+-. 10 ng/ml for 4-hydroxypropranolol and 9 .+-. 2 ng/ml for propranolol glycol. These plasma levels were less than the peak concentrations recorded for propranolol (123 .+-. 34 ng/ml). The rates of plasma decay of these 2 metabolites were greater than that observed for propranolol.