The Primary Structure of Alanine Transfer Ribonucleic Acid 1 from Torulopsis utilisII. Partial Digestion with Ribonuclease T1 and Derivation of the Complete Sequence
- 1 August 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 74 (2) , 323-333
- https://doi.org/10.1093/oxfordjournals.jbchem.a130250
Abstract
Partial RNase T1[EC 2.7.7. 26] digestion of tRNAAla1(Torulopsis utilis) in the presence of Mg2+ yielded two half molecules by scission at the anticodon site. Several large oligonucleotides smaller than the halves were obtained by partial RNase T1 digestion of the tRNA in the absence of Mg2+. The sequences of these large fragments were analyzed. The complete sequence of this tRNA was derived from the results of these analyses and those described in the preceding paper. The cloverleaf model for the secondary structure was compared with that of Saccharomyces tRNAAla, as regards the aminoacyl-tRNA synthetase recognition sites.Keywords
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