The photoaffinity probe 8‐N3[α‐32P]ATP labels the ATP‐binding sites of rabbit neutrophil and skeletal muscle actin
- 8 August 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 159 (1-2) , 145-149
- https://doi.org/10.1016/0014-5793(83)80434-7
Abstract
8-Azido-[α-32P]ATP (8-N3-ATP) was used as a photoaffinity label for ATP binding sites in the subcellular fractions of rabbit peritoneal neutrophils. The radioactive 8-N3-ATP was specifically incorporated into one major protein of 43 kDa. The isoelectric point, molecular mass and subcellular distribution of this labeled protein closely resemble those of the actin. 8-N3-[α-32P]ATP was further tested as a photoaffinity label for the ATP binding site in the purified rabbit skeletal muscle G-actin. The radioactive 8-N3-ATP was specifically incorporated into the actin band in SDS—polyacrylamide gel. The results indicate that 8-N3-ATP can be used as a photoaffinity label for actinKeywords
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