QUANTIFICATION OF APOC-II AND APOC-III OF HUMAN VERY LOW-DENSITY LIPOPROTEINS BY ANALYTICAL ISOELECTRIC-FOCUSING

  • 1 January 1978
    • journal article
    • research article
    • Vol. 19  (8) , 1047-1052
Abstract
ApoC-II and apoC-III of human very low density lipoproteins (VLDL) were quantified by analytical isoelectric focusing between pH 4-6 in polyacrylamide gels containing 8 M urea. The isoelectric point of apoC-III0 is pH 4.93; apoC-II, pH 4.78; apoC-III0, pH 4.72, and apoC-III2, pH 4.54. ApoC-I is not found in the pH range between pH 4-6. Two minor peptides, apoC-IV and apoC-V, with isoelectric points of pH 4.61 and 4.44, respectively, are apoproteins not previously identified. The sensitivity (5-40 .mu.g) and reproducibility (.+-. 8%) of this method allow quantitative analysis of apoC-II and apoC-III distribution in VLDL.